AU - Chaudhry, Gul-e-Saba AU - Akim, Abdah AU - Zafar, Muhammad AU - Abdullah, M AU - Sung, Yeong AU - Muhammad, Tengku TI - Induction of apoptosis and role of paclitaxel-loaded hyaluronic acid-crosslinked nanoparticles in the regulation of AKT and RhoA PT - ORIG DP - 2018 Jul 1 TA - International Journal of Orthodontic Rehabilitation PG - 101-106 VI - 9 IP - 3 4099- https://www.orthodrehab.org/article.asp?issn=2349-5243;year=2018;volume=9;issue=3;spage=101;epage=106;aulast=Chaudhry;type=0 4100- https://www.orthodrehab.org/article.asp?issn=2349-5243;year=2018;volume=9;issue=3;spage=101;epage=106;aulast=Chaudhry AB - Cancer is a complex multifactorial disease and leading causes of death worldwide. Despite the development of many anticancer drugs, there is a reduced survival rate due to severe side effects. The nontargeted approach of convention drugs is one of the leading players in context to toxicity. Hyaluronan is a versatile bio-polymer and ligand of the receptor (CD44) on cancer cells. The MCF-7 and HT-29 cancer cell lines treated with hyaluronic acid-paclitaxel (HA-PTX) showed the distinguishing morphological features of apoptosis. Flow cytometric analysis showed that HA-PTX induces apoptosis as a significant mode of cell death. The activation level of tumor suppressor protein (p53) increased after PTX treatment in MCF-7, but no changes observed in HT-29 might be due to hereditary mutations. The lack of suppression in AKT and Rho A protein suggest the use of possible inhibitors in future studies which might could play a role in increasing the sensitivity of drug towards mutated cells line and reducing the possibilities for cancer cell survival, migration, and metastasis.